Building on and Integrating Previous Knowledge   - by Ann Burgess Example of a Worksheet   - prepared by Lynn Allen-Hoffmann Team No. ___________    Include only those present     Facilitator: ______________________     Monitor: ______________________     Recorder: ______________________     Reporter: ______________________   Worksheet for Week 9 Research Paper and Technical Comments: Chen, Y. Chen, C.-F., Riley, D.J., Allred, D.C., Chen, P.-L., Von Hoff, D., Osborne, C.K., Lee, W.H. (1995) Aberrant subcellular Localization of BRCA1 in Breast Cancer. Science 270:789-791. Scully, R., Ganesan, S., Brown, M., DeCaprio, J.A., Cannistra, S.A., Feunteun, J., Schnitt, S. Livingston, D.M. (1996) Technical Comment: Location of BRCA1 in Human Breast and Ovarian Cancer Cells. Science 272:123-125. Chen, Y., Chen, P.-L., Riley, D.J., Lee, W.H., Allred, D.C., Osborne, C.K. (1996) Response: Location of BRCA1 in Human Breast and Ovarian Cancer Cells. Science 272:125-126. 1A. In the original Chen et al. report, in Figure 2B,C, and D, the authors determined the localization of BRCA1 in normal and breast cancer cells. How was this accomplished? 1B.The authors used several controls in this experiment. List four controls and the experimental purpose of each. 2A. What data is conspicuously missing from Figure 2A in the original Chen et al. report? Question 2 is continued on the back. 2B. In the technical comment, Scully and coworkers conducted a similar analysis (Figure 3) to that in Figure 2A of the original Chen report. Compare and contrast the data represented in these two figures from the different research groups. 3. In their reply to the technical comments of Scully and coworkers, how did Chen et al. directly address the problems associated with cross-reactivity of the antibody reagents and fixation procedures? 4. In your opinion, what additional information was learned about BRCA1 location/mislocation in breast cancer cells as a result of the technical comment articles? Explain your reasoning. 5. Suggest here a question to be discussed on April 2 by the panel of women who have had breast cancer. The question should relate the research you have been learning about in this unit to the lives of women affected (or potentially affected) by breast cancer. Tell me more about this plan: A general description Some advice and reflections on this plan Why I like this activity Example of a study guide Example of a quiz Example of a worksheet Example of a team evaluation How much time does this take? More about Ann Burgess

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